Establishment of the Monoclonal Antibodies against Plasmodium falciparum Fcc7801/HN
- VernacularTitle:抗恶性疟原虫Fcc7801/HN株单克隆抗体的制备
- Author:
Chunlei JIANG
;
Weibin GUAN
;
Xiaoli WANG
;
Xiantao KONG
- Publication Type:Journal Article
- Keywords:
Plasrnodium falciparum;
antibodies, monoclonal;
immunoblotting fluorescent antibody tedmic
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Four cell lines (C6, H6, B6, 2H6) of hybrids secreting monoclonal antibodies (McAb) against erythrocytic stage of Plasmodium falciparum Fcc7801/HN were established by fusing SP2/0 myeloma cells with spleen cells of BALB/c mice immuniaed with the preparation of the same malaria parasite and cloning three times. Of these four lines, C6 and B6 showed significant growth-inhibition of P. falciparum in vitro. Both C6 and B6 McAb recognized 71 kD antigen of P. falciparum by Western Blotting. The results indicate that the 71 kD antigen is of immune protection The four McAb showed cross immunofluorescence reactivity with P. falcipanum Fcc-1/HN, Fcc7802/HN, Fcc8703/JS and P. berghei. P. cynomolgi. It indicates that there are common epitopes among these malaria parasites, and that it is important in immunologic research and serodiagnosis of malaria
