Determination of transcription activity of rat liver nucleic free RNA Polymerase in vitro
- VernacularTitle:大鼠肝细胞核游离型RNA聚合酶的体外转录活性测定
- Author:
Junze LIU
;
Lukang YI
- Publication Type:Journal Article
- Keywords:
RNA Polymerases;
transcription;
genetic;
rat;
actinomycin
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The inhibitory effects of actinomycin D on chromatin (or DNA) template and the stimulating effects of synthetic template Poly [d(A-T)] on transcription were observed in the liver nucleic transcriptional system of the rat in vitor.By introducing proper concentrations of actinomycin D and Poly [d(A-T)] into each reactionary system,a method to assay free RNA polymerase activity in the nucleus was established.In addition,a comparison of the contents between chromatin-engaged and free polymerase in normal rat liver nuclei isolated with hypertonic sucrose (2.3 mol/L) was made.Eventually,the possible role free RNA Polymerases play in the eukaryotic transcriptional process was discussed.
