Comparison of Monoclonal Antibody J28 and Peanutagglutinin Immunoblotting for Detecting Fetoacinar Pancreatic Protein
- VernacularTitle:单克隆抗体J28免疫印渍与花生凝集素印渍检测胚胰腺泡蛋白的比较
- Author:
Wenjun ZHANG
;
Guoming XU
;
Zhiqun ZHOU
;
Hongju ZHANG
- Publication Type:Journal Article
- Keywords:
fetocinar pancreatic protein;
monoclonal antibody J28;
peanutagglutinin;
immunoblotting
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
We used SDS-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting of monoclonal antibody (McAb) J28 and peanutagglutinin (PNA) for detecting fetoacinar pancreatic protein (FAP) in extracts of 20 cases of fetal pancreas, 5 of pancreatic cancer and 4 of normal pancreas. The result showed that fetal pancreas and pancreatic cancer had a same level band about 110000 in Commassie blue stain, but absent in normal pancreas. The band could react with both McAb J28 and PNA PNA also could react with smaller glycoprotein in pencreatic cancer. It is suggested that McAb J28 and PNA combine with different sites of FAP.
