A study of precancerous gastric lesions with mucohistochemistry, immunohisto chemistry and cell DNA quantitative analysis
- VernacularTitle:胃粘膜癌前病变的粘液组化、免疫组化及流式细胞DNA定量的研究
- Author:
Chunqi LI
;
Weiwen LIU
;
Baiou WANG
- Publication Type:Journal Article
- Keywords:
precancerous conditions;
stomach neoplasms;
DNA;
mucuc
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
One hundred and sixty-eight specimens of precancerous lesions of gastric mucosa were studied with mucohistochemistry,and ABC immunohisto-chemistry stains of MG7 which is a monoclonal antibody against human gastric cancer (GC).In addition,quantitative analysis of cell DNA was performed with flowcytometry in 36 specimens.It was found that the incidence of type I b intestinal metaplasia (IM) was significantly higher in dysplasia and in the mucosa adjacent to GC than in chronic atrophic gastritis (CAG),and the positive rate of MG7-Ag in type Ⅱb IM was significantly higher than that in type Ⅰa,Ⅰb and Ⅱa IM.Type Ⅱb IM could degenerate into dysplasia.MG7-Ag positive rate,DNA aneuploid rate and the percentage of S and G2+ M cells were significantly higher in type Ⅱb IM with dysplasia than in simple type Ⅱb IM.These findings support the supposed progressive course of CAG →type Ⅱb IM→dyspalsia→GC,and type Ⅱb IM with dysplasia is closely associated with GC.
