Effect of pH on Binding of Purine Nudeotides to Uncoupling Protein in Subnitochondrial Parti-cles from Rat Brown Adipose Tissue
- VernacularTitle:pH对大鼠棕色脂肪组织亚线粒体片段上解偶联蛋白与嘌呤核苷酸结合的影响
- Author:
Xuemin WANG
- Publication Type:Journal Article
- Keywords:
brown fat;
mitochondria;
uncoupling agents;
purine nucleotide;
hydrogen-ion concentration
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Using submitochondrial particles, from rat brown adipose tissue mitochondria treated by Lubrol and 3 mol/L urea, as a model, the relationship between different pH and pKd of binding of purine nucleotides to uncoupling protein was estimated. When it was shown as plots of pKd against pH, it was found that the pKd peak of binding of GDP or ADP to uncoupling protein was about pH 5.5. The greater pH deviated, the less pKd became. The highest pKd of binding of GTP or ATP to uncoupling protein was also near pH 5.5, below which pKd remained constant, but above which pKd dropped rapidly. At a certain pH, pKd of binding of GDP or GTP to uncoupling protein was higher than that of binding of ADP or ATP, respectively.
