STUDY ON THE INTERACTIONS BETWEEN TETRAMETHYLPYRAZINE PHOSPHATE AND LIVER MICROSOMES IN RATS
- VernacularTitle:磷酸川芎嗪与大鼠肝微粒体相互作用的研究
- Author:
Feng LI
- Publication Type:Journal Article
- Keywords:
Tetramethylpyrazine phosphate ( TMPP );
Phenobarbetal ( PB );
Liver microsome;
Cytochrome P-450
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
The two methods of preparing rat liver microsomes were compared. The microsome yield by ultracentrifugation was far grater than that by Ca2+ aggregation(P 0.05) . The results showed that the ig treatment of TMPP at the doses of 50, 100 and 300 mg/kg, respectively, once daily for 10d, had no significant effects on the liver weight, microsome yield, cytoch-rome P-450, Cytochrome b5 and NADPH Cyt C reductase. It was indicated that TMPP metabolized by liver had no ability to induce or inhibit the cytochrome P-450 enzymes in rats. Pretreatment with phenobarbital (75mg/kg?d-1, for 3d ) , a mainly P-450b inducer, significantly decreased the plasma concentrations of TMPP in rats. Therefore, it is suggested that the metabolism of TMPP in rats is probably related to the cytochrome P-450b of liver.
