EFFECT OF TOTAL GLUCOSIDES OF PAEONY ON LEUKOTRIENE B_4 BY PERITONEAL MACROPHAGES IN RATS
- VernacularTitle:白芍总甙对大鼠腹腔巨噬细胞产生白三烯B_4的影响
- Author:
Jun LI
;
Weizhong ZHAO
;
Minzhu CHEN
;
Shuyun XU
- Publication Type:Journal Article
- Keywords:
Leukotriene B_4;
Paeonia Lactiflora Pall;
Total gluco-sides of paeony;
Peritoneal macrophages;
RP-HPLC
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Total glucosides of paeony ( TGP ) , an extract of Paeonia Lacti-flora Pall were investigated for its effect on the synthesis of leukotriene B_4 ( LTB_4 ) by peritoneal macrophages ( PM?). LTB4, generated by 10~9~15 ? 10~10/L PM? with ionophore A_23187 , were measured with RP-HPLC. Suppression of releasing LTB_4 from PM? by TGP ( 100 mg/L ) was as much as the same dose of Flufenamic acid, but the action of TGP was slower. TGP significantly inhibited the synthesis of LTB_4 by PM? in a dose-dependent manner. The concentration of TGP required to obtain 50% inhibition ( IC_50 ) of formation of LTB_4 was 0.66mg/L. The data suggested antiinflam-matory and immunomodulatory action of TGP were related to its suppression of releasing LTB_4
