THE USE OF THE INDIRECT ELISA IN THE DETERMINATION OF ANTIENDOME- TRIAL ANTIBODIES AND ITS CLINICAL APPLICATION
- VernacularTitle:酶联免疫吸附间接法检测抗子宫内膜抗体及其临床应用
- Author:
Qiuchao HUANG
;
Gongnan ZHUANG
;
Jianguo WU
;
Qi LIU
- Publication Type:Journal Article
- Keywords:
ELISA;
Endometrial antibodies
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The endometrial antigens from human endometrium were purified by collagenase (Type I), 250um, 40um, sieves and affinity chromatography. Indirect ELISA was used to detect antiendometrial antibodies (EMAb). The findings showed that the positive rates of EMAb in this assay were 43.4% (infertile, n=129), 37.1% (abortion, n=113), 6.7% (middle and old man, n = 120), 3.8% (pregnant woman, n = 52) and 0% (male, n = 43), respectively.
