Ultrastructural Study of Human Epidermal Keratinocytes Cultured in Low Calcium Medium
- VernacularTitle:在低钙培养液中培养的人表皮细胞的超微形态观察
- Author:
Fangping DAI
;
Junlong LIU
;
Yulin CHEN
;
Wenzheng WANG
- Publication Type:Journal Article
- Keywords:
human epidermal keratinocyte culture;
low calcium medium;
proliferation and differentiation;
ultrastructure
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A low calcium medium developed for epidermal keratinocytes were prepared according to the MCDB 153 modified formula and used in human epidermal keratinocyte culture compared with DMEM culture system. The observation by contrast microscopy and electron microscopy showed that in the low calcium medium keratinocytes grew as a monolayer of high proliferation and had many characteristics of basal cells, with a more rounded shape and large intercellular spaces. Increasing the calcium ion concentration in the medium or changing the other culture conditions the cells in these cultures could be induced stratification and terminal differentiation. The results suggest that the growth, proliferation and differentiation of cultured human epidermal keratinocytes can be controlled and regulated someway.
