Experimental study Detecting Entroviruses RNA With the Method of cDNA: RNA Spot Hybridization
- VernacularTitle:用cDNA:RNA斑点杂交法检测肠道病毒RNA的实验研究
- Author:
Qian SHEN
;
Meixiang WANG
;
Tonghua ZHANG
- Publication Type:Journal Article
- Keywords:
biotin probe;
enterovirus;
spot hybridization
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Taking Coxsackie B3 virus cDNA labelled by Biotin as detecting probes and 25 kinds of standard enteroviruses as detected viusr, a spot nucleic acid hybridization method has been established to detect enterovirus RNA gene. The method has simple operation and its sensitivity is 50-80 TCID50 spot. Reagent is safe and stable, and may be stored for long period. pCBHⅢ/35.51 probe used has wider hibridization spectrum to enteroviruses; pCBⅢ/29 only reacts with RNA of Coxsackie B3 virus in strict condition. Consequently, the hybridization character which these probes possess is suitable to determining enlerovirus in clinical samples.
