Effect of 1, 25 (OH)_2D_3 Combined with Low-dose Chemotherapeutant on Leukemic Cells
- VernacularTitle:1,25(OH)_2D_3联合小剂量化疗药物对白血病细胞的体外作用
- Author:
Tao CHENG
;
Fang YAN
- Publication Type:Journal Article
- Keywords:
antineoplastic agents;
1, 25 (OH)2D3;
harringtonine;
daunorubicine;
HL-60 cells
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of 1, 25 (OH)2D3 in combination with low-dose chemotherapeutant on the human promyelocytic leukemic cells (HL-60) were investigated. In 4-day-cultured cells, 1, 25 (OH)2D3 and low-dose harringtonine (HH) or daunorubicine (DNR) had synergic effect in inducing differentiation of HL-60 cells, and HH or DNR significantly potentiated the effect of 1, 25 (OH)2D3 in inhibiting the cell proliferation, which were demonstrated by NBT reduction test and [3H]-TdR incorporation, respectively. Cell cycle analysis by flow cytometery revealed that the cells in S phase decreased remarkably and most cells stopped at G0/G1 phase. The results provide a new way for the inducers in treatment of leukemia.
