Studies on the Teratogenic Potential Effects of the Volatile Oil of Dacus carat L. Seed in Sprague-Dawley Rats in Vitro and in Vivo

VernacularTitle:野胡萝卜籽挥发油碳氢部位对SD大鼠体内和体外的潜在致畸胎作用
Publication Type:Journal Article
Keywords: volatile oil of Daucus carol L. seed; rats; teratogenesis
From: Academic Journal of Second Military Medical University 1985;0(05):-
CountryChina
Language:Chinese
Abstract: The teratogenic potential effects of the volatile oil of Daucus carat L. seed were studied in Sprague-Dawley rats with in vitro embryo midbrain and limb bud cells culture and in vivo conventional teratological experiment. All of 77 pregnant rats and 703 fetuses were examinated in vivo. The compound was administered into the embryo cells and pregnant rats at the doses of 50, 100 and 150?g/ml in vitro and of 625.0, 312.5 and 156.3mg/kg in vivo, respectively. Fish liver oil and normal saline were separately used for the positive and negative control groups. The results showed that the compound did not inhibit the differentiation of embryo midbrain and limb bud cells, and had no toxic effects on maternity, embryo and fetal growth for any treatment group. Based on these data, both tests in vitro and in vivo suggest that the volatile oil does not show the teratological effects in Sprague-Dawley rats, and the result in vivo is closely consistent with that in vitro.