Hepatic mitochondrial dysfunction and lipid peroxidation after and radiation-burn injuries in mice
- VernacularTitle:放射损伤、烧伤及复合伤时肝线粒体功能障碍与脂质过氧化作用
- Author:
Ye XIONG
;
Zongrong CHEN
- Publication Type:Journal Article
- Keywords:
radiation injury;
burn injury;
lipid peroxidation;
antioxidant;
cytochrome oxidase;
mitochondrium
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Male mice were subjected to 6 Gy total body irradiation,20% TBSA full thickness burns,or both the injuries respectively,and then lipid peroxides (LPO),vitamin E,sulfhydryl groups,respiratory control ratio (RCR),ADP/O ratio,and cytochrome oxidase activity of hepatic mitochondria were determined in the animals in the first 9 postinjury days.It was found that.1.LPO level increased in the early postinjury stage in the combined radiation-burn injury group.2.Vitamin E level decreased significantly in both the groups with either only irradiation or burns.3.The sulfhydryl groups showed a tendency to increase in all the 3 groups.4.The activity of cytochrome oxidase increased signficantly on the 7th postinjury day in the combined injury group.5.RCR and ADP/O ratios decreased more significantly in the combined injury group than in either the single injury group.These facts suggest that the suppression of the respiratory function of hepatic mitochondria results from the damage of mitochondrial membranes due to iipid peroxidation.
