Effects of Derivatives of Human Glycophorin A on the Recognition and Invasion of Plasmodium falic parum into Erythrocytes
- VernacularTitle:人血型糖蛋白A衍生物对恶性疟原虫裂殖子识别及入侵红细胞的效应
- Author:
Xiangdong WANG
;
Xunyi LUO
;
Jianxin LI
;
Yaomei WEI
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum merozoite;
human glycophonn A;
erythrocyte
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Electrophoretic-purified human glycophorin A (GPA) was used to produce its derivatives: (1) glycopeptides were separated and purified from GPA by trypsin digestion; (2) preparation of GPA-antibody and GPA glycopeptide-antibody; (3) preparation of deglycosylated GPA (dGPA); (4) incorporating GPA or dGPA into human RBC membrane lipids to form two kinds of liposomes. The products described above were used to test Plasmodium falciparum FCC1/HN merozoites for their ability to invade human erythrocytes. It was found that GPA-liposomes were able to bind with merozoites and dGPA-liposomes had a negative reaction. GPA, GPA glycopeptide, GPA-antibody, GPA glycopeptide-antibody and GPA-liposome all had the effect to hinder the invasion of merozoites into human erythrocyte while dGPA-liposome had no such an effect.
