Relationship of Human AFP Variant Peptide and Carbohydrate Difference and Clinical Diagnosis of Primary Hepatocellular Carcinoma
- VernacularTitle:人甲胎蛋白异质体肽链和寡糖成分差异与肝癌诊断的关系
- Author:
Baihe ZHANG
;
Mengchao WU
;
Xiaohua ZHANG
;
Han CHEN
;
Xiangfu WU
- Publication Type:Journal Article
- Keywords:
alpha-fetoprotein;
variant;
primary hepatic cancer;
clinical diagnosis;
lentil lectin
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AFP from fetal serum and serum of patients with primary hepatic cancer were purified by affinity chromatography. Then, the lentil lectin-reactive and nonreactive variants of these purified glycoproteins were prepared by affinity chromatography with immobilized lectin. Glycopeptides and oligosaccharides were prepared from variants by protease treatment and hydrazinolysis, respectively, and subjected to carbohydrate and amino acid analysis. A small difference in the carbohydrate compositions of each variant was observed. Analysis of chemical compositions of AFP variants is useful for the differential diagnosis between benign liver diseases and primary hepatic cancer.
