Effects of Low-dose Daunorubicin on Proliferation and Differentiation of HL-60 cells
- VernacularTitle:小剂量柔红霉素对HL-60细胞增殖分化的影响
- Author:
Tao CHENG
;
Fang YAN
- Publication Type:Journal Article
- Keywords:
daunorubicin;
low-dose chemotherapy;
leukemia;
HL-60 cells;
proliferation;
differentiation
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to probe into the mechanism of low-dose chemotherapeutic agents in the treatment of leukemia, we studied the effects of low concentrations (l-40ng/ml) of daunorubicin (DNR) on proliferation and differentiation of human promyelocytic leukemia cell line (HL-60). 1 ng/ml DNR could inhibit the proliferation of the cells, but no induction of differentiation was found. DNR at more than 5 ng/ml had both cytotoxicity and induction of differentiation. Through the count of survival cells, [3H]TdR and [3H]UR incorporation, cloning efficiency, cell morphology and NBT reduction test, it is concluded that low-dose DNR could have inhibitory and cytotoxic effects on the cells, and its inducing differentiation was not important.
