Preliminary Study of Partial cDNA Cloning for Human Clotting Factor Ⅷ
- VernacularTitle:人凝血因子Ⅷ基因cDNA的部分分离的初步研究
- Author:
Jun TAN
;
Xinfen QIU
;
Houg LIANG
- Publication Type:Journal Article
- Keywords:
human clotting factor VIII;
DNA sequence;
cloning
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A cDNA library prepared from human liver was screened for Factor Ⅶ,a clotting factor in the middle phase of blood coagulation,with P114.12 probe.3 recombinant phages were distinguished,and one of the 3recom-binants was further analyzed and extended by 450 bp as compared with the p114.12 probe.The clone 1 (FⅧhc1) was sequenced and homology between 210 bp of FⅧhc1 and 5559-5769 in FⅧ cDNA was found.It was shown that the FⅧhc1 extended forwardly 800~900bp from 17 exon of FⅧ cDNA.
