EFFECT OF 1?, 25-DIHYDROXY-VITAMIN D_3 ON DIFFERENTIATION AND PROLIFERATION OF A HUMAN MYELOID LEUKEMIA CELL LINE
- VernacularTitle:1?,25二羟基维生素D_3对人早幼粒白血病细胞(HL—60)的诱导分化作用
- Author:
Yan XUE
;
Jie TIAN
;
Hongxia WANG
- Publication Type:Journal Article
- Keywords:
1 a,25-Dihydroxy-vitamin D3;
Induced differentiation;
Human myetoid leukaemia cell line
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
1 ? 25-dihydroxy-vitamin D3(1? 25 (OH)2 D3)induced differentiation of human promyelocytic leukaemia cells ( HL-60 ) into mature myeloid cells in vitro. The ratio of nuclei to cytoplasma decreased; Their nucleoli reduced; Cells became irrgular and extended the pseudopods. 1 ? 25 (OH ) 2 D3 caused significant increase of nitroblue tetrazolium ( NBT ) reduction and ?-non-specific acid esterase (?-NAE ) and acid phosphatase (ACP ) activities. These data and morphological characteristics suggest that HL-60 cells were conclusively identified as monocytes/macrophages. The histogram of DNA distribution ahylyzed by flow cytometry demonstrated G0 + G1 phase increased and S phase increased and S phase decreased remarkably after treatment with l?, 25 ( OH ) 2 D3 as compared with untreated cells.
