Determination of Globin Beta-chains and Comparison of Genetic Type of SMMC/B Mice of Three Generations by Peptide Map Analysis
- VernacularTitle:三代SMMC/B小鼠珠蛋白?肽链结构分析与遗传型比较
- Author:
Shukui SONG
;
Kaiyuan HU
- Publication Type:Journal Article
- Keywords:
globin;
betanchain;
fingerprint;
amino acid sequence;
inbred strain of mice;
genetic type
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The separation and purification of beta chains of globm from the inbreeding SMMC/B mice were performed by the dissociation of chains in urea, CMhcellulose column chromatography for the separation of alpha and beta chains, and Sephadex G25 column chromatography for removing salts. The pure beta-chain was digested by trypsin. Digested fragments were resolved by cellulose membrane two-way electrophoresis, thus obtaining the map of spots of beta-chain fragments for B33, B34, Bp(), Bp and Bf. The spots,BTp3, which have the same positions ,were separated and micro-sequences were assayed by DABITC/PITC double coupling technique. According to the genetic code, we might deduce the nucleotide sequence of their mRNA from the known amino acid sequence of BTp3, a piece of beta-chain. By analyzing the altered genetic loci of beta-chain of globin, we might determine the genetic purity of the strain and distinguish objectively the genetic types between species and subspecies.
