Observation of Hepatocellular Ultrastructure and G-6-Pase Cytochemistry in Hepatocarcinogenesis Induced by Diethylnitrosamine in Rats
- VernacularTitle:二乙基亚硝胺诱发大鼠肝癌肝细胞超微结构及葡萄糖—6—磷酸酶细胞化学的观察
- Author:
Yonghi YAN
;
Yuanyao XIA
;
Xuting YE
;
Wenming CONG
;
Mengchao WU
;
Xiuhong ZHANG
- Publication Type:Journal Article
- Keywords:
hepatocytes;
ultrastructure;
G-6-Pase activities;
diethylnitrosamine;
rats
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In the process of hepatocarcinogenesis induced by diethylnitrosamine (DENA) in rats, the hepatocellular ultrastructure and G-6-Pase reactions in hepatic nodes were observed by electron microscope. The results are as follows: As compared with normal hepatocytes, cell junctions were fewer, even disappeared in some areas and intercellular spaces were wider; in some cells, nuclear membranes invaginated into the nucleoplasms frequently, micleoli were enlarged, mitochondria appeared swollen and their cristae were scanty- and short, and depolymerized ribosomes dropped off the dilated rough endoplasmic reticulums; in some seriously diseased cells, nucleoli were enlarged, abundant free ribosomes were present, but the' other organelles were in lower differencial state. G-6-Pase reactions were positive before the 8th week of DENA induction and negative after the 12th week. These suggest the hepatocellular metabolic disturbance and low differenciation.
