A STUDY OF ENZYME-CONJUGATED SYNTHETIC DNA PROBE FOR THE DIAGNOSIS OF FALCIPARUM MALARIA
- VernacularTitle:合成酶联DNA探针对恶性疟病人的检测
- Author:
Shuzhen CHEN
;
Zhaosong ZHANG
;
Zhen LIN
;
Rongzi WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Schistosomiasis Control
1989;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A 21-base, Plasmodium falciparum specific, enzyme-conjugated synthetic DNA probe (PFRl-AP) was used for the diagnosis of falciparum malaria. The blood samples (53 P. falciparum, 5 P. vivax, and 3 P. f and P.v mixed infection cases ) collected from Hainan province were tested. The samples of 32 college students were used for normal control. The probe proved to be specific and sensitive. 10pg of purified P.f DNA could be always detected, and there was no cross reaction with the purified DNA of human leukocytes. When testing Hainan blood specimens, PFRl-AP specifically detected P.f infections. In dot blot, when Nytran membrane with 50 microliters of treated blood samples being used, 39 out of 52 P.f specimens hybridized with this probe positively. When the volume of blotted sample was increased to one hundred microliters, the accumulative total positive rate rose up to 88.46%. The samples of P.v and normal control showed negative reaction with this probe.
