The Harmful Effect of Prostaglandin on Peripheral Nerve Regeneration and the Action of Its Synthesase Inhibitor Indomethacin
- VernacularTitle:前列腺素及消炎痛对周围神经再生的影响
- Author:
Taoyuan XIAO
- Publication Type:Journal Article
- Keywords:
nerve regeneration/DE;
prostaglandins/PD;
indomethacin/PD;
radioimmunoassay
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The present experimental study was carried out to investigate the effect of indogenous prostaglandeins produced in the peripheral nerves after injury and the action of indomethacin ( a prostaglandin synthesis inhibitor) on their regeneration on the animal models with sciatic nerve crushed. The main results were: 1 , Radioimmunoassay demonstrated a stepladder-like increase in PGF 2 alpha in the sciatic nerve after crushing injury ; 2 , light microsocpy showed the number, diameter and area of the cross section of regenerated, myelinated nerve fibers passing through the crushed segment of the tibial fascicle of sciatic nerve of the rabbits being more than those injected with normal saline ; 3 , Culture of dissociated dorsal ganglia root cells from newborn rabbits revealed that nerve cells grown in media containing PGF 2 alpha showed significantly smaller size, less number of regenerated processes and lower content of RNA than those grown in media containing indomethacin. Scanning electronmicroscopy demomstrated different surface morphological features. These results indicated that an excess prostaglandin was produced in the rcushed nerve and inhibited nerve regeneration. Indomethacin as a prostaglandin synthesis inhibitor prevented excessive prostaglandin formation to promote nerve regeneration.
