Effects of Burn Patient's Sera and Fibrinopeptide A and B on Endothelial Cells
- VernacularTitle:烧伤血清、纤维蛋白肽A、B对血管内皮细胞的影响
- Author:
Jing AN
- Publication Type:Journal Article
- Keywords:
burns/PP;
blood/AN;
fibrinopeptides A;
fibfinopeptides B;
endothelium
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Endothelial injury appears very early after traumatic and toxic shock. The mechanism and etiology are-complex.The low-molecular weight fibrinogen degradation-products (LmwFDP)may be one of the important factors.Dynamic changes of fibrinopeptide A and B (FPA, FPB) in sera of burn patients (BPS) are determined with HPLC method. On this basis detrimental effects of BPS and FPA, FPB on endothelial cells are observed in vitro. The main results are as follows: 1) after being incubated with BPS or the sera with complements being inactivated by heatine at 56 degrees C for 30 min, the endothelial cells (EC) all become denatured and necrotized, and the levels of 6-keto-PGF1? and LDH in the media are higher than those measured before incubation. This indicates that the presence of the factors other than complements, in the BPS injurious to the cultured EC; 2) FPA and FPB in 18 BPS become increasing at 2 postburn day (PBD) , reach their peaks at 5-7 PBD, then decline after 15 PBD,and return to the control levels at 25 PBD.The fluctuation of FPA,FPB levels in BPS are parallel to the progress of burn illness. The measureements of FPA, FPB levels in BPS may be useful in evaluating patients condition. 3) FPA and FPB are detrimental to the cultured EC in vitro. Characteristics of their injurious effects are:inreversible,dose dependent,and specific. The FPA and FPB may play an important role in endothelial injury.
