Analysis of the Components of Cell-free Preparationsof Human Fetal Liver and Its Effect on Survivalof Experimental Hepatic Failure
- VernacularTitle:无细胞人胎肝制剂的成分及其对大鼠肝衰竭的疗效观察
- Author:
Like WU
- Publication Type:Journal Article
- Keywords:
liver diseases/TH;
cytosol, fetal liver;
cytosol,fetal muscle;
cytosol, adult liver, human;
tissue extracts/TU;
galactosamine
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Effect of cell-free preparation of human fetal liver (cytosol and its dialysa-te) , human fetal muscle cytosol(FMC) and human adult liver cytosol (ALC) on survival rate of rats with acute hepatic failure induced by D-galactosamine was studied. The components of the 4 preparations were analysed preliminarily. Results: 1 ,fetal liver cytosol(FLC) could increase the survival rate(SR) of rats with hepatic failure(HF) comparing with normal saline(P0.05) . The SR of rats with HF was reduced by ALC markedly) 2,IgG,IgA,IgM and C3 could not be detected in FLC, but a few kinds of hormones, trace elements, glucose and lipids, and biological large molecule substances (BLMS) such as nucleic acids and proteins were found. As to free amino acid,content of branch chain amino acids in FLC was significantly higher than that in ALC, but the content of aromatic amino acids and ammonia was markedly lower than that in ALC.The components in FLD were almost the same as in FLC except BLMS.These data suggest that the soluble BLMS in FLC may play a key role in treating HF. ALC could reduce SR and contained a large amount of poisonous substances such as aromatic amino acids and ammonia.
