EFFECTS OF CALCIUM-REGULATOR HORMONES ON PROSTAGLANDIN METABOLISM AND THE MECHANISMS OF BONE RESORPTION
- VernacularTitle:调钙激素对前列腺素代谢的影响及对骨吸收作用的机制
- Author:
Fengyun HAN
;
Jinxuan CHENG
;
Jinhong DUAN
;
Zhengang WANG
- Publication Type:Journal Article
- Keywords:
Parathyroid hormone;
Caleitonin;
Mononuclear cell;
Cyclic AMP;
Prostaglandin E_2;
Prostaglandin E_1
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Lymphokine-osteoclast activating factor(OAF) is a potent mediator of bone resorption. PGs which mediate bone resorption are necessary for OAF. It has been reported that mononuclear is an important factor in bone resorption; and circulating monocytes may be osteoclast precursors,but it is not clear at present. We used CT and PTH which are potent physiological calcium-regulator hormones to stimulated mono-nuclear to study it's role for bone resorption. Mononuclear was isolated from normal human peripheral blood by Ficoll-Hypaque. After cultured in C02 incubator at 37℃ for 24h. 0.05g/L PTH and 100U/L CT were added and incubated in the same condation. After 2.5min, 5min, 10min, and 15min cAMP was measured by RIA. In the sam way PGE was determined. Our experiment exhibited that circulating mononuclear responds to CT and PTH with an increase in cAMP firstly, and enhances PGE production by CT and PTH stimulated mononuclear secondly. The results suggest that mononuclear participated in the regulation of bone resorption by PGE, OAF and other factor.
