HEPATIC STIMULATORY SUBSTANCE (HSS) FROM THE LIVERS OF NEW-BORN CALF AND RATS
- VernacularTitle:新生牛和大白鼠肝脏中肝刺激物质的研究
- Author:
Ming XIE
;
Fang GU
;
Yanan WANG
;
Keming JIA
- Publication Type:Journal Article
- Keywords:
Hepatic stimulatory substance DNA-synthesis Liver cell proliferation
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The HSS found in the cytosol of new-born calf liver was heat-stable and it promoted the DNA-synthesis of guinea-pig liver cells (P/o:5.72),HSS was also found in the cytosol of new-born rat livers,and its most effective concentration to promote incorporation of H2-TdR into DNA of guinea-pig liver cells was about 0.05mg/ml.This activity was reduced with both the increase and decrease of cytosol content,and the reason was not clear.It was proved with SDS-PAGE that the molecular weight of the active component was about 14000 and 23000 daltons.The cytosol from control rat livers showed no HSS activity and also no component measuring 23000 daltons.It needs further identification that the 23000 daltons protein is the active component in the cytosol of new-born calf and rat livers and the partially hepatectomized rat livers,but not in the cytosol of control rat livers.This finding indicateds that the livers of new-born calf and rat contain highly active HSS and are a valuable resource of HSS
