The Preparation and Application of Anti-human Heterogeneous Lens culinaris Agglutinin Reactive AFP Monoclonal Antibodies
- VernacularTitle:抗人小扁豆凝集素结合型甲胎蛋白异质体单克隆抗体的研制和初步应用
- Author:
Baihe ZHANG
- Publication Type:Journal Article
- Keywords:
Lens culinaris agglutimn;
AFP;
monoclonal antibody;
heterogenecity
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, we report the study concerning the preparation and application of anti-human heterogeneous AFP-R-LCA monoclonal antibodies (VG5 VD12 VB5 VA8 VD12). These McAbs had higher affinity and specificity to AFP-R-LCA than the routinely used anti-AFP polyclonal antibody. Competitive assays indicated that they could bind to new antigenic determinants which are different from the previously reported a and b sites. In an assay of the McAbs, we first set up a two site sandwich ELISA method and then tested, using this method, serum samples from 69 patients with PHC, 67 patients with benign liver diseases, 30 pregnant women and 30 normal controls. The results showed that this two site sandwich ELISA method had low pseudo-positive rate. The detection range was 5 1000 ng/ml, with the properties of simplicity, accuracy and reproducibility. It could be used not only for the early diagnosis of PHC in patients with AFP levels less than 400 ng/ml, but also for the differential diagnosis of PHC from benign liver diseases.
