Effects of 1,25-Dihydroxyvitamin D_3 on Inhibition of Proliferation and Induction of Differentiation of HL-60 Cells
- VernacularTitle:1,25(OH)_2D_3对HL-60细胞的抑制增殖和诱导分化作用
- Author:
Fang YAN
- Publication Type:Journal Article
- Keywords:
1,25-dihydroxyvitamin D3;
HL-60 cells;
inhibition of proliferation;
induction of differentiation
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
Inhibition of proliferation and induction of differentiation by 1, 25-dihydroxyvitamm D3 of human promyelocytic leukemic cell line (HL-60)are reported. At the concentration range of 109 to 106 mol/ L, 1, 25-dihydroxyvitamin D3 had effect in inhibiting cell proliferation with time-dependent and dose-dependent manner. Strong inhibition of colony cell growth (74.8?11.1%)was observed at 107 mol/L concentration. It was also found that HL-60 cells could be induced into monocytes by 1, 25-dihydroxyvitamin D3at the same concentrations,which were demonstrated by cytochemistry and specific antigen of cell surface and NBT reduction test. In addition, the relationship between proliferation and differentiation is discussed according to the result of flow cytometry.
