METHOD FOR DETERMINATION OF TOTAL GLUTATHIONE
- VernacularTitle:总谷胱甘肽的测定方法
- Author:
Yiming XIA
- Publication Type:Journal Article
- Keywords:
glutathione plasma DTNB
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The method of recirculating assay for total glutathione is presented in this paper. The principle is that the concentration of total glutathione is proportional to TNB-forming rate resulting from the reaction between GSH and DTNB provided that NADPH and GR are used to keep the amount of total glutathione constant. It is very difficult to get accurate concentrations of glutathione in human plasma because of the very low concentration and the extremely fast disappearance after the blood was drawn. These difficulties are overcome by means of fast measurement and using y~GT inhibitor. The sensitivity of this method is about 0.1 uM and the recovery is 93-106%.
