In Situ Hybridization and RNA Dot Blot Analysis of Alpha-fetoprotein Messenger RNA in Human Hepatocellular Carcinoma and Its Host Liver
- VernacularTitle:用原位杂交和RNA斑点杂交法检测人肝细胞癌和癌旁肝中甲胎蛋白mRNA
- Author:
Linseng YANG
- Publication Type:Journal Article
- Keywords:
in situ hybridization;
dot blot hybridization;
hman hepatocellular carcinoma;
alphafetoprotein;
messenger RNA;
biotinylated probe
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
The expression of the alpha-fetoprotein in 12 cases of human hepatocellular carcinoma and its host liver by in situ hybridization analysis of AFP mRNA in cryostat and paraffin - embeded tissue sections was studied. Total RNA was extracted in 3 cases of cancer tissues and its host liver tissues for RNA spot blot Both the tumor cells and the surrounding host liver cells showed positive hybridization with a biotinylated AFP cDNA probe. AFP mRNA existed in almost all tumor cells, but the distribution pattern of AFP mRNA in the host liver varied. The positive cells were dispersed in the tissue or formed foci or appeared to be a small piece of positive area. Our results showed that not only the tumor cells can synthesize AFP, but also the surrounding liver cells have the capacity to produce AFP.
