Establishment of the Hybridomas Secreting Monoclonal Antibodies against Rabbit ALD-A and Its Primary Application
- VernacularTitle:分泌抗ALD-A单克隆抗体杂交瘤细胞株的建立与初步应用
- Author:
Cunzhong WU
- Publication Type:Journal Article
- Keywords:
monoclonal antibody;
ALD-A;
hcpatocellular carcinoma
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
The ALD-A from rabbit muscle was employed to immunize BALB/c mice. After hybridation and selection, five hybridomas secreting high titer anti-ALD-A monoclonal antibodies were prepared, among which the antibody from hybridoma E10 was found to be IgM. It was also found that this antibody did not reacted with normal human liver, spleen, lung, stomach and colon tissues but reacted weakly with normal human muscle. On the other hand, the human hcpatocellular carcinoma tissues were stained by PAP whate-ver the sera of these patients were AFP positive or negative. The results suggest that monoclonal antibodies directed against ALD-A might have potential application in diagnosis of hepatocellular carcinoma especially those with serum AFP negative.
