Regulative Action of Substance P on Interferon-? Production in Mouse Spleen Cells Induced by Concanavalin A and Pokeweed Mitogen
- VernacularTitle:P物质对刀豆素A和美洲商陆诱导小鼠脾细胞产生?干扰素的调节作用
- Author:
Yeping TIAN
- Publication Type:Journal Article
- Keywords:
substance P;
interferon-?;
concanavalin A;
pokeweed mitogen
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The influences of different concentrations (10-11-105mol/L) ofsubstance P(SP)on the interferon -?(IFN-?) production in mouse spleen cells induced by concanavalin A (ConA) and pokeweed mitogen (PWM) in vitro were studied. The results showed that no influence of 10-11-10-6 mol/L SP on the titre of IFN-? induced by PWM (10?g/ml) was observed; the titres of IFN-? induced by ConA or PWM (5, 2 5 and 1. 25 ?g/ ml) were enhanced by 10-9-10-5 mol/L SP; this enhancement was more significant at 10-7-10-5 mol/L SP. No titre of IFN-? was detected in the presence of SP alone. The titres of IFN-? induced by ConA added at 6h after adding 10-9-10-6 mol/L SP were slightly higher than that induced by ConA and 10-9-10-6 mol/L SP added simultaneously. The titre of IFN-? induced by ConA and 10"5 mol/L SP added in advance tended to reduction rather than enhancement as compared with that induced by ConA and 10-5 mol/L added simultaneously. The effect of enhancing IFN-? production began to appear significantly at lower concentration (10-8 mol/L) of SP added in advance, whereas at 10-7 mol/L SP added simultaneously.
