HPLC Determination and Phaimacokinetic Investigation of Propafenone in Heahhy Volunteers
- VernacularTitle:高效液相色谱法测定正常人普罗帕酮血浓度及药动学参数
- Author:
Gaolin LIU
- Publication Type:Journal Article
- Keywords:
propafenone;
HPLC;
plasma concentration;
pharmacokinetics
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A RP-HPLC method was described for the determination of plasma propafenone. The column was 25 cm?4. 0 mm ID stainless steel packed with ODS (5 ?m). The mobile phase consisted of CH3OH: HAC-NaAC: H2O (70: 15: 15, v/v), containing 0. 0584 mol/L diethylamine as a modifier. The flow rate was 1. 0 ml/min. Tetracaine was used as a internal standard. Chromatography was performed at 250 run with variable wavelength ultraviolet detector. The propafenone was first extracted from alkalized plasma with 2.0 ml of 2% (v/v) isoamyl alcohol in n-heptane, and then reextracted with 0. 3 mol/L H3PO4, 100?l. Linear calibration curve for propafenone was measured over the range of 50-1600 ng/ml, and correlation coefficient was 0. 9993. The recovery rate of the method was 88.82?6.39% (n =5), and the coefficient of variation was less than 10% (n = 10). The lowest determined level was 40 ng/ml.The pharmacokinetic parameters were measured by this method in 5 healthy volunteers after a single oral dose propafenone 300 mg. The mean pharmacokinetic parameters were as follows: t1/2 (Ka=0. 40?0. 15 h, t1/2 (K) =2. 81?0. 52 h, Tmax= 1. 88?0. 47 h, Cmax= 631. 67?453. 13 ng/ml, and AUC= 3843.06?3032. 80ng?h-1?ml-1..
