CRYOPRESERVATION OF MURINE BONE MARROW CELLS AND THEIR TRANSPLANTATION
- VernacularTitle:深低温保存小鼠骨髓细胞及其移植效果
- Author:
Zhenhai DING
- Publication Type:Journal Article
- Keywords:
cryopreservation;
transplantation;
chalone;
bone marrow
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Murine bone marrow cells were cryopreserved in liquid nitrogen with programmable freezing system as a source of hematopoietic cells to transplant into irradiated recipients for the treatment of hematopoietic failure. The results showed that CFU-GM in graft was slightly affected by freezing with a recovery of 75.7%, but CFU-S which act as major role in hematopoietic recons-titution was not obviously injuried (recovery of 126.7%). In the transplantation study, the frozen-thawed bone marrow cells gained survival rate of 43.6% which was slightly higher than obtained by fresh cells, suggesting that cryopreserved bone marrow cells still have intact hematopoietic reconstructive ability. For the purpose of preventing graft-versus-host disease (GVHD), chalone, an extract from calf spleen, was used in our studies and inhibited the early GVHD effectively.
