Relationship between Somatostatin Neurons and Vasopressin or Neurotensin:A Double Labeling Electromicroscopic Study
- VernacularTitle:大鼠下丘脑室旁核和室周核生长抑素神经元与加压素或神经降压素的共存——PAP与胶体金双标记免疫电镜观察
- Author:
Ying QIN
- Publication Type:Journal Article
- Keywords:
hypothalamus;
somatostatin;
vasopressin;
neurotensin;
immunohistochemistry.
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to demonstrate the relationship between somatostatin (SRIF) and vasopressin (VP) or ncurotensin (NT) in periventricular nucleus (Pv) and paraventricular nucleus (PVN) of the rat hypothalamus, pre-embedding PAP labeling SRIF combined with post-embedding colloid gold labeling VP or NT was used in this study. The results presented that some of SRIF-immunoreactive cells were VP positive. SRIF and VP co-contained dendron was observed between the subendymal cells. SRIF and VP double labeled axon made synaptic contact with SRIF and VP double labeled perikarya and other unstained dendrons. In the Pv and PVN, coexistence of SRIF and NT in the same neurons was revealed. Its axon made synapse with negative dendron. In Pv, SRIF axon made synaptic contact with SRIF and NT co-contained dendron. Some of SRIF-immunoreactive cells were not labeled by VP or NT. These results suggested that the interregulation between SRIF and VP or NT might occur intracellularly. The two kinds of double labeled neurons were involved in local nucleus modulation. The SRIF and VP co-contained neurons may receive information and secrete hormone into cerebrospinal fluid.
