Preliminary Study of Immunodiagnosis of Trichinosis by ELISA Using Urea Soluble Antigen of Trichinella spiralis
- VernacularTitle:尿素溶解性抗原用于ELISA诊断旋毛虫病的初步研究
- Author:
Jinli ZHANG
;
Fuchun LAI
- Publication Type:Journal Article
- Keywords:
trichinosis/DI;
ELISA( = enzyme-linked immunosorbent assay);
immunoenzyme technique;
antigens;
Trichinella sipralis
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Urea soluble antigens of Trichinella spiralis infective larva were prepared and the efficiency of the antigens in diagnosis of trichinosis by ELISA are studied. Specific IgG antibodies are detected by ELISA using urea soluble antigens in 91.67%(22/24) of 24 sera and 91.67% (ll/12)of 12 blood spots of filter paper of patients of trichinosis. Specific IgM antibodies are seen in 95.24% (20/21) of 21 sera of patients using the antigens. Sera of patients infected with Schistosoma japonicum have some cross reactions on the antigens. Sera from mice infected with - Schistosoma japonicum, Ancyclostoma caninum larva, and Plasmodium yoelii are negative. The urea soluble antigen has a relatively high sensitivity, specificity and good reproducibility in diagnosis of trichinoisis by ELISA, and has a practical and economic value.
