THE QUANTITATIVE ANALYSIS OF FRACTIONS OF GLUCOSE OLIGOSACCHARIDES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- VernacularTitle:高效液相色谱法测定葡萄糖低聚糖的组分
- Author:
Huaichun WU
;
Hua CHENG
;
Yi LU
- Publication Type:Journal Article
- Keywords:
oligosaccharide glucose oligosaccharides glucose polymers high performance liquid chromatography
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The contents of the individual fractions of glucose oligosaccharides were determined simultaneously by means of reversed-phase HPLC. The sample was dissolved in water and filtered and the filtrate was used directly for the analysis. The sugar was separated on a column of u-Bondapak C18 using water as the mobile phase and determined refractometrically.The method was applicable to the analysis of glucose oligosaccharides from monomeric glucose through polymeric maltooctose. Quantitative results were obtained for glucose, maltose, maltotriose, maltotetrose, maltopentose and maltohexose in the samples prepared in our laboratory. Recovery tests revealed this method reliable.
