ESTABLISHMENT OF COXSACKIE B_5 VIRUS INFECTED RAT HEART CELLS IN CULTURES
- VernacularTitle:柯萨奇B_5病毒感染心肌细胞微量模型的建立
- Author:
Qian SHEN
- Publication Type:Journal Article
- Keywords:
Coxsackie B3 virus;
myocardial cells;
cell culture
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A micro-model of Coxsackie B5 virus infected rat beating heart cells was introduced.In the infected group, the cardiac enzymes-lactic acid dehydrogenase (LDH) and glutamic oxaloacetic transaminase(SGOT)werc significantly increased and the synthesis rate of DNA was decreased 48 h after inoculation with 400 TCID50 Coxsakie B6 virus.Virus titer(Lg 6.95 TCID50)in the supernatant of the infected cultures was detected 96 h after virus challenge.In addition, the specific fluorescence in cytoplasma of infected cells could be observed.These results suggest that Coxsackie B5 virus might be replicated, assembled and released in cultured rat myocardial cells.
