PREPARATION AND STRUCTURAL CHARACTERIZATION OF THE LIPID A COMPONENT OF SALMONELLA TYPHIMURIUM SL1102 RE-LIPOPOLYSACCHARIDE
- VernacularTitle:鼠伤寒SL 1102菌粗糙型内毒素类脂A的制备及化学结构分析
- Author:
Binghua JIAO
- Publication Type:Journal Article
- Keywords:
S.typhimurium SL 1102;
lipopolysaccharide (endotoxin);
lipid A;
chemical and structural analysis;
13C and 31P-nuclear magnetic resonance
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The lipid A component of Re-lipopolysaccharide from S.typhimurium SL 1102 was prepared by hydrolysis with a 0.1 mol/L acetate buffer.Chemical as well as advanced l3C-,and 31P-NMR investigations revealed a ?1' ,6-interlinked D-glucosamine diasaccharide, which is substituted by two phosphate(P)groups, one being esterlinked to the non-reducing residue,and another bound glycosidically to the C1 of the reducing residue, is as the central backbone.Its structure is shown as follows:-P-GlcN(?1', 6)GlcN-P-The amino(2 and 2')and hydroxy(3 and 3')groups of the backone are substituted by 3-OH-14: 0, and unhydroxy fatty acids appear to be the subtituents of the hydroxy group of 3-OH-14 :0, in the means of formation of 3-acyloxyacyl ester and amides.
