DETERMINATION OF PANTOTHENIC ACID IN INFANT FORMULA BY HPLC
- VernacularTitle:高效液相色谱法测定婴儿奶粉中的泛酸
- Author:
Jingshan ZUO
;
Lijuan ZHANG
- Publication Type:Journal Article
- Keywords:
pantothenic acid high performance liquid chromatography
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The determination of pantothenic acid in infant formula was carried out by means of HPLC. The sample was dissolved in deionizid water and put in an ultra-sonic generator for 15 minutes, adjust pH to precipitate protein. After centrifugation the supernatants were used as test sample for HPLC analysis. Column: Bondapak C18, Detector: ultraviolet 200nm, and 0.01M KH2PO4/CH3OH as eluant. Coefficient of variation was 6.58% and recovery was 93.78%.The method might be applicable to milk and milk products and alsoto the samples containing free pantothenic acid such as wheat germ and flour.
