METHODS OF QUANTITATIVE IMMUNOCYTOCHEMICAL STUDY OF THE HYPOTHALAIVIIC VP-LIKE SUBSTANCE IN CONTROL AND FOOT SHOCKED RATS WITH MICROSCOPIC PHOTOMETER
- VernacularTitle:未标记酶免疫细胞化学染色的半定量显微分光光度测定法
- Author:
Yi DING
- Publication Type:Journal Article
- Keywords:
quantitative immunocyiochemistry;
non-labelled enzyme method;
microscope photometer
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Here are reported for the first time two newly established methods of semi-quantitative measurement of specific antigens by means of n microscope photometer in the tissures stained using non-labelled enzyme immunocyio-chemistry-the point and area scanning methods. We measured the content of vassoprcssin-like material in the vassopressinergic neurons of hypothalamus in the control and foot shocked rats with the two methods,respectively. The results prove them, to some extent, significant in studying the content of specific material in specific tissues (e.g. some nuclei), and even in single cells. In addition, a comparison between the two methods was conducted in this study.
