EXPRESSION OF ?B-IFN GENE IN E. COLI
- VernacularTitle:?B型干扰素基因在大肠杆菌中的表达
- Author:
Zhaorong WEN
- Publication Type:Journal Article
- Keywords:
interferori;
gene expression;
rccombinant plasmid;
cytopathogenic inhibition assay
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The recombinant plasmids containing IFN-?B gene fragments were constructed by ligating IFN gene fragment trimmed by Bal-31 exonclease and vector pUR222 digested by HincⅡ . In some of the recombinant plasmids ?B-IFN gene was able to be expressed under the control of lac. promoter of pUR222. Over 700 transformants were screened by cylopathogenic inhibition assay in microplate. Fifteen transformants showed antiviral activity,and one of them, pSM2317 reached 1?108 U/L.
