DETERMINATION OF GLUCOCORTICOfD RECEPTORS IN RAT SPLENIC LYMPHOCYTES
- VernacularTitle:大鼠脾淋巴细胞糖皮质激素受体的测定
- Author:
Feng LI
- Publication Type:Journal Article
- Keywords:
splenic lymphocytes;
glucocorticoid receptor;
receptor
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The method for measuring glucocorticoid hormone receptors in rat splenic lymphocytes was developed. The results showed that there were specific binding sites of 3H-Dexamethasone with high affinity (Kd : 5,05?1.70 nmol/L), low binding capacity (Bm3s or R0 : 6773?585 specific binding sites/cell) and tempera- ture-dependent nuclear translocation in the rat splenic lymphocytes. This paper provides a convenient method for study of the molecular mechanisms of glucocor-ticoid hormone action and pathophysiology of glucocorticoid hormone receptors.
