Studies of the Effects of Hydrogen Peroxide on the Ultrastructure and Permeability of Candida Albicans
- VernacularTitle:过氧化氢对白色念珠菌超微结构及通透屏障影响的研究
- Author:
Xiaofeng YU
;
Ying TU
- Publication Type:Journal Article
- Keywords:
Hydrogen peroxide/pharmacodynamics;
Candida albicans/drug effect;
Candida albicans/ultrastructure
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The Effects of hydrogen peroxide(H2o2) on the ultrastructure, cellular pea-meability barrier and 3H-TdR-DNA leakage of C. albicans were studied. Damage of the cell, especially leakage of cytoplasm were found under transmission electron microscope. Morphological changes and small hole like structures were also found under scanning electron microscope. Radioactivity assay of the leakage of 3H-TdR-DNA leakage of C. albicans further suggested that the 3H-TdR-DNA leakage of C. albicans was directly due to the damage of cellular permeability barrier. Negative correlation (r=+0.8392--0.9915)between the extent of 3H-TdR-DNA leakage and the number of surviving colony-forming units was found. This indicated that the damage of cellular permeability barrier and leakage of DNA of C. albicans caused by H2o2 was one of the most importent causes of cell killing.
