DETECTION OF HBcAg WITH NITROCELLULOSE-ENZYME LINKED IMMUNO- SORBENT ASSAY (NC-ELISA)
- VernacularTitle:硝酸纤维素膜酶联免疫吸附法检测乙型肝炎病毒核心抗原
- Author:
Fangqiu LI
- Publication Type:Journal Article
- Keywords:
NC-ELISA HBcAg
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
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Abstract:
A new ELISA method for the detection of HBcAg in serum has been established with nitrocellulose as the solid carrier.HBV-anti-HBs complex formed with patient's serum and horse anti-HBs was applied to the nitrocellulose membrane under vacuum, using a special apparatus, and then was fixed on the filter at room temperature over night. HBcAg was determined with HRP-anti-HBc after treatment with 3mol/L NaCNS. Comparison with the methods for serum HBc Ag previously described, this technique was simple in performance. Its sensitivity and specificity were satisfactory for clinical purpose. The results from the study of clinical specimens indicated that HBcAg was closely correlative to HBsAg, anti-HBc and HBV-PHSAR.
