A STUDY ON THE IMMUNOLOGICAL FUNCTION OF PATIENTS SUFFERING FROM GASTRIC OR ESOPHAGEAL CANCER BY MEANS OF MONOCLONAL ANTIBODIES la AND OKT SERIES
- VernacularTitle:用la和OKT单克隆抗体研究胃癌和食管癌患者的免疫功能
- Author:
Zhongbing ZHANG
- Publication Type:Journal Article
- Keywords:
Immunological function Gastric cancer Esophageal cancer Monoclonal antibodies. Ia,OKT series.
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
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Abstract:
By means of monoclonal antibodies la and OKT series it is possible to classify B and T lymphocytes as well as the subgroups of T cells, thus making appraisement of the immunological function of the patients possible. Hereby we presented the results of observation among 17 cases of esophageal can-c er, 20 cases of gastric cancer, in comparison with 17 normal persons. All of the cancer patients, were diagnosed with the aid of endoscopic and pathohistologic examinations. Lymphocytes were separated from fasting blood samples. Immunofluorescent stainings were performed, using mouse anti-human monoclonal antibodies Ia,OKT3, OKT4, OKT8 and rebbit antimouse IgG fluorescent antibody. The cells, exhibiting fluorescent dots on the cell membrane are counted and their percentage among the tatal lymphocytes is calculated.In normal persons T lymphocytes (OKT3 positive) accounS for 74.03 .19% of total lymphocytes. in the peripheral blood, T helper lymphocytes (OKT4 positive) 49.02 +3.88%,T suppressor lymphocytes (OKT8 positive)28.98?.98%, B lymphocytes (la positive) 20.86?.98%. The average ratio between T helper cells and T suppressor cells or OKT4/OKTS ratio is 1. 72. In the 17 cases of esophageal cancer the average percentages of T helper cells and T suppressor cells are 41.2715.98% and. 40.09?.11%, respectvely, with the OKT4/OKT8 ratio of 1.10. In the 20 cases of gastric cancer.the corresponding figures are 39.33?.29%, 36.26?.21% and 1.15, repectively. It demonstrats that in cancer patients there are a marked decrease of T helper cells and a marked increase of T suppressor cells and a consequent decrease of the OKT4/OKT8 ratio, indicating the presence of immunological disturbance.
