THE EFFECTS OF ACTINIDIA CHINENSIS PLANCH ON THE PREVENTION OF CANCERIII. The Blocking Effect of Fruit Juice on the Formation of N-Methyl-N'-Nitro-N- Nitrosoguanidine in VitroAmes Test
- VernacularTitle:中华猕猴桃的防癌作用(三)在模拟人胃液中对N-亚硝酰胺合成的阻断作用——Ames试验
- Author:
Puju SONG
;
Lian ZHANG
- Publication Type:Journal Article
- Keywords:
Actinidia chinensis Planch Ames test N-methyl-N'-nitro-N-nitrosoguanidine
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
A model system which simulated the conditions of the human stomach was used in the experiments. Precursors NaNO2 and MNG formed MNNG and resulted in the positive mutagenic response to Salmonella typhimurium TA 100. When the concentration of the precursors was 50 mM NaNO2 and 100 mM MNG, the induced average number of revertant per plate was 4327 showed an approximate twenty fold spontaneous revertants. With diluting of the precursors, the mutagenic response was lower, showing a dose-response relationship between the mutagenic activity and the precursors concentration. It was found that, when precursor concentration was lower than 22.2 mM NaNO2 and 44.4 mM MNG, the formation of MNNG was blocked completely by the Chinese Kiwi fruit juice and mutation was inhibited. In the same concentration without the Chinese Kiwi fruit juice, the induced revertant was an approximate 13 fold of the spontaneous revertant. The Chinese Kiwi fruit could not block the formation of the MNNG when the precursors concentration was higher than 33.3 mM NaNO2, but it inhibited the mutagenic activity partly. Compared with the Chinese Kiwi fruit juice, the effect of the ascorbic acid solution in the same concentration was much less.It was demonstrated with TLC that MNNG was formed in the model system.
