PINGYANGMYCIN-INDUCED HeLa CELLULAR DNA BREAK- AGE IN VITRO
- VernacularTitle:平阳霉素对HeLa细胞DNA的体外断裂作用
- Author:
Tianrong JIANG
;
Yusheng WANG
- Publication Type:Journal Article
- Keywords:
Pingyanyniycin;
Bleomycin A8;
DNA breakage in vitro;
Agarose gel electrophoresis;
Basic protein mounting technique
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Pingyangmycin was produced by Pingyangnesin sp No. 74 which was isolated from mud at Pingyang, Zhejiang China. The structure and properties of Pingyangmycin are the same as those of bleomycin A-g. Pingyangtnycin-induced cellular DNA breakage was observed in this experiment. DNA breakage in vitro was found by agarose gel electrophoresis and UV fluoresene photography after ethidium bromide staining 0. 5ug cellular DNA incubated with a series of drug concentrations for 2h and with a series of drug exposure times at a dose of 200 ug/ml. The mininal concentration and exposure time of DNA breakage is 5 ug/ml and 10 min. DNA molecule was observed under electron microscope with basic protein mounting technique. The natural spiral DNA long strand (X =5.95 um+1.95)was cut down into short DNA fragments ("X =0.75 n.m+0.24)by 10 ug/ml drug for 2h_ The length of untreated DNA is significantly longer than that of the drug treated one(P
