RAPID PURIFICATION OF WHEAT GERM AGGLUTINSN FROM WHEAT GERM
- VernacularTitle:从麦胚中快速分离纯化麦胚凝集素
- Author:
Shimin ZHANG
- Publication Type:Journal Article
- Keywords:
wheat germ agglutinin;
ovomucoid-Sepharose 4B;
polyacrylamjde gel electrophoresis
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Wheat germ agglutinin(WGA)with a higher activity of hemagglutination was purified from wheat germ by a modified method of ovomucoid-Sepharose 4B affinity chromatography.The average yield of purified WGA from 100g wheat germ acetone powder was 58.74?6.56mg. WGA showed a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with an apparent molecular weight of 34400.The specific haptens of WGA- were N-acetylglucosamine and sialic acid.
