Effects of Para-chlorophenylalanine on Corticosterone Receptor Binding Capacity of Hippocampal Cytosol in Adrenalectomized Rats
- VernacularTitle:对氯苯丙氨酸对大鼠海马胞液皮质酮受体数量的影响
- Author:
He YAN
- Publication Type:Journal Article
- Keywords:
Hippocampus;
Corticosterene;
Receptor;
5-HT;
Para-chlorophe-nylalanine;
Corticosterone;
Receptor radioligand binding assay
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The properties of the corticosterone receptors and the effects of para-chlorophenylalanine(pCPA) on the binding capacity of the corticosterone receptors of the hippocampal cytosol in adrenalectomized rats were investigated with receptor radioligand binding assay(RLA) using (3H)-corticosterone as the ligand. It was fonud that the corticosterone receptors possessed the properties of satu-rability, limited binding capacity, high affinity and high specificity. RLA demonstrated a linear Scatchard plot, which implies that there is only one receptor or one group of receptors with similar Kd values. Four days after intracerebroven-tricular injection of 1 mg of pCPA, the hippocampal concentration of 5-HT was markedly decreased, and the binding capacity of the cytosol receptors was increased, which implies that the functional activity of the serotoninergic system in the hippocampus is an important factor in the control of the number of corticosterone receptors in rats.
